Composite

Part:BBa_K3544105:Design

Designed by: 博宇 邹   Group: iGEM20_SCU-China   (2020-10-22)


NLS-yeGFP-CRISPR(Csy4)-DsRed


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1387
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1387
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1387
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1387
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1387
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 696


Design Notes

Lead yeGFP and DsRed into nucleus and cytoplasm, respectively

For more imformation, please view https://2020.igem.org/Team:SCU-China/Design.